Midwall ejection fraction for assessing systolic performance of the hypertrophic left ventricle
© Yoshikawa et al.; licensee BioMed Central Ltd. 2012
Received: 9 October 2012
Accepted: 4 November 2012
Published: 20 November 2012
In patients with left ventricular hypertrophy (LVH), LV midwall fractional shortening (FS) is used as a measure of LV systolic performance that is more physiologically appropriate than conventional FS. For evaluation of LV volume and ejection fraction (EF), 2-dimensional (2D) echocardiography is more accurate than M-mode echocardiography. The purpose of this study was to assess systolic performance by midwall EF using 2D speckle tracking echocardiography (STE).
Sixty patients were enrolled in the study. Patients were divided into two groups with LVH (n = 30) and without LVH (control group, n = 30). LV systolic function was compared between the two groups and the relationships of left ventricular mass index (LVMI) with LV systolic parameters, including midwall EF, were investigated.
Midwall EF in the LVH group was significantly lower than that in the control group (42.8±4.4% vs. 48.1±4.1%, p <0.0001). Midwall FS was also significantly lower in the LVH group (13.4±2.8% vs. 16.1±1.5%, p <0.0001), but EF did not differ significantly between the two groups. There were significant correlations between midwall EF and LVMI (r=0.731, p <0.0001) and between midwall FS and LVMI (r=0.693, p <0.0001), with midwall EF having the higher correlation.
These results show that midwall EF can be determined using 2D STE. Midwall EF can be used to monitor LV systolic dysfunction, which is not possible with conventional EF. Evaluation of midwall EF may allow assessment of new parameters of LV systolic function in patients with LV geometric variability.
KeywordsMidwall ejection fraction Left ventricular systolic function Left ventricular hypertrophy Speckle tracking echocardiography
Left ventricular (LV) systolic performance is often assessed by ejection fraction (EF) and fractional shortening (FS). It is said that despite depression of LV systolic function, LVEF and LVFS are preserved in patients with left ventricular hypertrophy (LVH) [1–6]. However, assessment of LV systolic function at the endocardial surface is thought to reflect a geometric change of the LV, rather than the contractile function of the myocardium . Midwall FS has been used as a more physiologically appropriate measurement of LV systolic performance in patients with LVH, compared to conventional FS, and decreased midwall FS is predictive of subsequent morbidity and mortality [1–9]. However, calculation of midwall FS is based on a limited region of the LV, rather than the whole left ventricle. Therefore, evaluation of midwall FS for assessment of LV systolic performance may not be applicable in patients with variable LV geometries. Two-dimensional (2D) echocardiography is more accurate for evaluating LV volume and EF compared with M-mode echocardiography, and 2D speckle tracking echocardiography (STE) allows measurement of LV volume and EF without manual tracking [10, 11]. Therefore, the purpose of this study was to investigate the utility of midwall EF using 2D STE.
Population and study protocol
Sixty patients were enrolled in the study. Patients were divided into two groups with LVH (n = 30) and without LVH (control group, n = 30). LVH was defined as an LV mass index (LVMI) >96 g/m2 for women and >114 g/m2 for men. The causative of patients with LVH was essential hypertension with the exception of chronic renal failure, idiopathic hypertrophic cardiomyopathy, amyloidosis. Patients with myocardial infarction, atrial fibrillation, valvular disorders, and any other structural heart disease were excluded. Patients with poor echocardiographic image quality in the apical 2-chamber and 4-chamber views were also excluded. LV function was measured using conventional echocardiography, tissue Doppler imaging (TDI), and 2D-STE. LV systolic function was assessed by EF, midwall FS, midwall EF, and longitudinal strain. Systolic function was compared between the two groups and the relationships of LVMI with LV systolic parameters, including midwall EF, were investigated. Ethical review board approval from our hospital was obtained.
Midwall FS = (LVID d + H d /2) − (LVID s + H s /2)/(LVID d + H d /2).
Volume of myocardium between the midwall and the endocardium = (LVID d + H d /2)3 − LVID d 3 = (LVID s + H s /2)3 − LVID s 3.
H d = PWT + IVST.
where LVID is the LV internal dimension, d is end diastole, s is end systole, and H is the shell thickness. In diastole, the inner and outer shells have, by definition, equal thickness given by (PWT + IVST).
Tissue Doppler imaging
TDI was performed in all patients with images taken based on the guidelines of the American Society of Echocardiography . Using the 4-chamber view, a 5-mm sample volume was placed at the septal and lateral border of the mitral annulus. Annular velocities were displayed in septal and lateral pulsed-wave TDI and the early systolic mitral annular velocity (S’), the early diastolic mitral annular velocity (E’), and the late diastolic annular velocity (A’) were determined from the average of septal and lateral data from the TDI recordings. The mitral E/E’ ratio was also calculated.
Real-time 2D imaging
2D image analysis was performed on digitally stored images (X3 transducer, Philips iE33 system). Real-time 2D data sets were obtained from the apical 2-chamber and 4-chamber images. Images were recorded to allow for reliable operation of the software (Q-Lab, Version 7.0, Philips Healthcare).
LV systolic strain measurement
Myocardial longitudinal strain measurement was assessed on 4-chamber image with speckle tracking analysis. The traced endcardium is automatically divided into six segments; septal, anteroseptal, anterior, lateral, posterior, and inferior. The average peak strain measured in the longitudinal directions defined as the longitudinal strain.
Midwall EF measurement
These data were obtained using the same method as that for midwall EF measurement, except for the positions of ROIs, which were based on anatomic landmarks, including septal and lateral points on the mitral annulus and a point on the apical endocardium. The EF was then calculated from apical 4-chamber and 2-chamber views, as for the midwall EF in 2D STE.
Data are expressed as means ± SD. A Student t-test was used to compare continuous variables and a χ2 test was used for categorical variables. Simple linear regression analysis was used to evaluate relationships between variables of interest, with p < 0.05 considered to indicate significance.
Patient characteristics in the LVH and control groups
Systolic blood pressure (mmHg)
Diastolic blood pressure (mmHg)
Echo parameters in conventional echocardiography
Echo parameters in conventional echocardiography in the LVH and control groups
E velocity (m/s)
A velocity (m/s)
Systolic echo and TDI parameters
Systolic echo parameters in the LVH and control groups
Midwall EF (%)
Midwall FS (%)
Longitudinal strain (%)
Relationships between echo parameters and LVMI
Relationships between LVMI and echo parameters
Correlation coefficient (r)
The reproducibility of the EF and midwall EF measurements was evaluated by calculating the intra- and interobserver variability in 30 of the 60 patients selected at random. The intra- and interobserver variabilities were 4.1±3.3% and 4.8±3.2%, respectively, for EF, and 6.1±4.4% and 6.4±4.2%, respectively, for midwall EF.
The midwall EF in the LVH group was significantly lower than that in the control group and midwall EF was correlated with the degree of LVH. Our study showed the utility of midwall EF for assessing systolic performance of the hypertrophic left ventricle. Also, this method may be clinically useful and is likely to have low observer variability. As far as we are aware, there has only been one previous study of midwall EF. Jung et al. found that the midwall EF also discriminates the systolic function between patients with LVH and normal subjects , but the method used required manual tracing of echocardiographic images and a complicated calculation of echocardiographic data, which limits the clinical utility.
LVH is an independent predictor of adverse cardiovascular events in hypertension [9, 15]. Accurate assessment of cardiac function in patients with LVH is important in clinical practice. LV systolic function has been wildly assessed as the ratio of observed LV endocardial FS or EF to value predicted by the level of end-systolic stress in normal subjects . The degree of shortening and the level of opposing forces in myocardium in patients with LVH is different from normal subjects . The previous study reported that LVEF and LVFS are preserved in patients with LVH, despite depression of LV myocardial systolic function [1, 3–6]. In this study, the midwall EF in the LVH group was significantly lower than that in the control group and the correlation of LVMI with midwall EF was higher than that with any other parameters, including midwall FS. Thus, midwall EF can be used to monitor LV systolic dysfunction, which is not possible with conventional LVEF and LVFS.
Midwall FS has been used to detect depressed LV systolic function in patients with LVH [1, 3–5]. A previous study found significant differences in midwall measurements of the fiber shortening and lengthening velocities in normal and hypertrophic patients . The midwall FS measurement is preferred because systolic wall thickening is non-uniform, with the inner wall thickening to a substantially greater extent than the outer wall. This may be because fibers in the subendocardial and subepicardial myocardium are orientated longitudinally, whereas those in the midwall region are orientated circumferentially [4, 7]. Ishizu et al. also showed differences in radial strain between the inner and outer halves of the myocardium and differences in circumferential strain among the three layers in the endocardial, midwall, and epicardial myocardium by strain analysis using 2D STE . For these reasons, the LV midwall FS is a more physiologically appropriate measurement of LV systolic performance in patients with LVH, compared to conventional FS [1, 2, 4–9]. However, midwall FS measurements are inherently flawed because of foreshortening errors and reliance upon geometric models that may be inaccurate in the diseased heart . Also, echocardiographic calculation of midwall FS is a geometry-based index derived from linear measurement of the posterior and septal walls, and consequently cannot distinguish between septal and posterior wall function . Therefore, calculation of midwall FS is made from a limited region of the LV. In contrast, midwall EF can estimate foreshortening without use of a geometric model because midwall EF is calculated in planes. Thus, measurement of midwall EF is a relatively new approach that can mitigate the errors inherent in midwall FS.
The correlation of LVMI with midwall EF was higher than with TDI parameters. It has been suggested that TDI can be used to quantify regional ventricular function objectively and the mitral annular velocity may be a more sensitive index of LV function . However, TDI is particularly affected by translational and tethering effects, and angle-dependency. Therefore, there is some limitation in interpretation of measurements by TDI. In contrast, analysis of midwall EF is relatively free of the influence of these adverse affects. Thus, in a clinical setting, measurement of midwall EF is effective for quantifying the impairment of LV function due to LVH. It is reported that the longitudinal strain is a useful method for assessing myocardium systolic dysfunction in patients with LVH . Nonetheless, the correlation of LVMI with midwall EF was higher than with longitudinal strain in our study. Midwall EF measurements may be superior to detect myocardial systolic dysfunction in patients with LVH than longitudinal strain.
Our study also showed the usefulness of 2D STE for measurement of midwall EF. The STE technique relies on tracking of natural acoustic markers in the myocardium from frame to frame throughout the cardiac cycle using a sum of absolute differences algorithm . Thus, the application of 2D STE method has been widely used in the study of subclinical or overt LV dysfunction . Evaluation of midwall EF by 2D STE does not require difficult or lengthy acquisition and offline reconstruction, which are impractical in routine clinical use. The 2D STE method also allows automatic measurements of LV volume to be performed without the need for manual tracings. We positioned the initial ROI manually on the midpoint of the wall thickness at the end of diastole only. Then, we automatically obtained the volume curve using a speckle tracking algorithm throughout the cardiac cycle. In systole, the initial ROI was not positioned at the midpoint of the wall thickness because the systolic thickening of the inner layer is larger than that of the outer layer. Ishizu et al. also proved this phenomenon using a speckle tracking method . Thus, our method is similar to the mathematical midwall mechanism reported in previous studies [1, 2, 4–9] and may be clinically useful and is likely to have low observer variability.
In this study, midwall EF correlated with the degree of LVH. Patients with LVH have intrinsic dysfunction in both systole and diastole. Our study showed that midwall EF can detect LV systolic dysfunction, which cannot be detected by conventional EF. This may be an important sign of LV dysfunction in patients with hypertension, which may not always be due to diastolic dysfunction, but can often be caused by systolic dysfunction, as assessed by midwall EF . Evaluation of midwall EF may allow assessment of LV systolic performance in patients with various LV geometries. Our method is relatively easy to apply in conventional echocardiography, with clinical settings similar to those for volume measurement by the routine biplane method.
Our methods demonstrated that the midwall EF is clinically useful for detecting the systolic function in addition to midwall FS, TDI, and strain. Midwall EF can detect LV systolic dysfunction, which cannot be detected by conventional EF. The intrinsic systolic dysfunction may affect predictive of subsequent morbidity and mortality in patients with LVH. Midwall EF will have possibilities to detect the beneficial change of intrinsic systolic dysfunction by medical treatment in clinical settings.
The current study has several limitations. First, we did not examine the influence of afterload, which may affect the midwall analysis. Second, the subjects did not constitute a consecutive series and were selected according to image quality. Finally, midwall EF using 2D STE is not theoretically the same as conventional midwall FS, which is needed to measure both systole and diastole.
The study showed that midwall EF can be evaluated using 2D STE. The midwall EF in the LVH group was significantly lower than that in the control group and midwall EF was correlated with the degree of LVH. This measurement can be used to detect LV systolic dysfunction, which cannot be detected by conventional EF. Thus, evaluation of midwall EF may allow assessment of new parameters of LV systolic function in patients with various LV geometries.
Left ventricular hypertrophy
Speckle tracking echocardiography
Left ventricular mass index
Tissue Doppler imaging
Interventricular septal thickness
Posterior wall thickness
Left ventricular end-diastolic diameter
Left ventricular end-systolic diameter
Left atrial dimension
- E velocity:
Peak early mitral flow velocity
- A velocity:
Peak late mitral flow velocity
Ratio of mitral E and A
The early systolic mitral annular velocity
The early diastolic mitral annular velocity
The late diastolic annular velocity
Regions of interests.
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