Early endothelial dysfunction in cholesterol-fed rabbits: a non-invasive in vivo ultrasound study.
© Drolet et al; licensee BioMed Central Ltd. 2004
Received: 01 July 2004
Accepted: 21 July 2004
Published: 21 July 2004
Endothelial function in hypercholesterolemic rabbits is usually evaluated ex vivo on isolated aortic rings. In vivo evaluation requires invasive imaging procedures that cannot be repeated serially.
We evaluated a non-invasive ultrasound technique to assess early endothelial function in rabbits and compare data with ex vivo measurements.
Twenty-four rabbits (fed with a cholesterol diet (0.5%) for 2 to 8 weeks) were given progressive infusions of acetylcholine (0.05–0.5 μg/kg/min) and their endothelial function was assessed in vivo by transcutaneous vascular ultrasound of the abdominal aorta. Ex vivo endothelial function was evaluated on isolated aortic rings and compared to in vivo data.
Significant endothelial dysfunction was demonstrated in hypercholesterolemic animals as early as 2 weeks after beginning the cholesterol diet (aortic cross-sectional area variation: -2.9% vs. +4% for controls, p < 0.05). Unexpectedly, response to acetylcholine at 8 weeks was more variable. Endothelial function improved in 5 rabbits while 2 rabbits regained a normal endothelial function. These data corroborated well with ex vivo results.
Endothelial function can be evaluated non-invasively in vivo by transcutaneous vascular ultrasound of the abdominal aorta in the rabbit and results correlate well with ex vivo data.
Keywordsendothelial function atherosclerosis cholesterol-fed rabbits ultrasound.
Endothelial dysfunction occurs early in the development of atherosclerosis. Historically, evaluation of endothelial function in small animals has been performed on isolated vessel segments, or vessels exposed by surgical procedures. Very few attempts were made to develop a method of analysis of endothelium-dependent relaxation in vivo[1–5]. In those studies, an invasive intravascular ultrasound approach was used. Correlation between results obtained in vivo and data on isolated arteries ex vivo was never assessed.
Non-invasive methods to study endothelial function in humans (e.g. ultrasound of the brachial artery) have been used for many years and have yielded an important amount of data [6–12]. Unfortunately this non-invasive technique has never been transposed to animal studies.
The objective of the current study was to assess the reliability of transcutaneous vascular ultrasound in order to evaluate endothelial function in vivo in rabbits and to compare this non-invasive method with results obtained ex vivo on isolated aortic rings.
Material and methods
Acetylcholine, nitroglycerin and sodium nitroprusside were from Sigma (Markham, ON, Canada). Angiotensin II and endothelin-1 peptides were acquired from Peninsula Laboratories Inc. (San Carlos, CA)
Animals and Study Design
Twenty-four male New Zealand White rabbits (3–4 kg body weight) were used in this study. Animals were treated in accordance to the Guide to the care and use of experimental animals published by the Canadian Council on Animal Care and the protocol was approved by the Animal Protection Committee of the Université Laval. Sixteen rabbits were divided in two groups (n = 8) and all animals were fed with standard rabbit chow supplemented with 0.5% cholesterol (w/w) (Harlan, Indianapolis, IN) for 2 or 8 weeks respectively. The other 8 animals received normal rabbit chow for eight weeks (normal controls). After 2 weeks, 8 randomly chosen cholesterol-fed rabbits were killed; the others were kept alive for an additional 6 weeks (total of 8 weeks of hypercholesterolemic diet) as for the normal control group.
When animals were sacrificed abdominal and thoracic aortas were excised and immediately rinsed in freshly prepared Krebs buffer in preparation for the ex vivo experiments. Plasma samples were drawn from the marginal ear vein every week and plasma cholesterol levels were determined using a commercially available spectrophotometric assay kit (Roche Molecular Biochemicals, Saint-Laurent, Canada).
In vivo evaluation of endothelial function by trans-abdominal ultrasound
Video sequences from the first 15 seconds (void volume) of drug infusion at baseline and between 40 to 60 seconds of drug infusion were digitized (Dazzle Video Creator, Dazzle Multimedia, Fremont CA) and stored on a computer for analysis (Figure 2). Still frames of the aorta from both the baseline and drug infusion (n = 5 each) synchronized to the beginning of the QRS and the respiratory phase were analyzed. The maximal diameter was measured (5 beats averaged) using the SigmaScan Pro software (SSSP Science, Chicago IL). Care was taken to measure the same segment for each beat using anatomic landmarks as a guide. The mean of 5 diameters for each image of the aorta was calculated. Vessel cross-sectional area was then calculated assuming the aortic section is circular using the formula: Area = π (D/2)2 where D is the diameter of the aorta. Area was expressed in percent of change from baseline. Inter and intra-observer variability was assessed on 10 randomly selected studies.
Ex vivo endothelial function evaluation in isolated rabbit aortic rings
At the end of the protocol rabbits were given a sub-lethal dose pentobarbital (25 mg/kg) and were sacrificed by exsanguination. The middle part of the descending thoracic aorta as well as the abdominal aorta were removed and dissected free of adhering fat and connective tissues. The aorta was placed in warm Krebs solution. Rings of 5 mm thickness were suspended in individual organ chambers filled with 5 ml of oxygenated Krebs (37C pH 7.4). The segments were connected to force transducers and any variations in force were recorded continuously (WIN-SMT software, PO-NE-MAH inc., Gould, Valley View, OH.).
Baseline contractile response was evaluated by a 30 to 60 minutes exposition to KCl (80 mM) where the rings were gradually stretched to a resting tension of 2 g until steady state was reached. Following this initial experiment, contractile capacity was further evaluated by exposing the rings to other vasoconstrictors. Briefly, when the rings had recovered their resting tension after the initial KCL exposure, they were exposed sequentially to cumulative concentrations of L-phenylephrine (PE, 10-9 to 10-5 M), angiotensin II (10-10 to 10-7 M) and endothelin-1 (10-9 to 10-6 M). Results were compared to the initial response obtained with KCL 80 mM.
Relaxation studies were performed after a precontraction with PE (10-6 M). Cumulative concentrations of acetylcholine (10-9 to 3 × 10-6 M) or sodium nitroprusside (10-10 to 3 × 10-5 M) were used. Sodium nitroprusside was used as a non-endothelial dependant vasodilator while acetylcholine evaluated the endothelial-dependant vasodilatation. Relaxation was expressed as a percent of change from the pre-contracted tension with PE.
Results are expressed as mean ± standard error of the mean (SEM). Differences between the various conditions in the in vivo endothelial function experiments were evaluated with an ANOVA for repeated measures using the Tukey's post hoc test to evaluate significance. In the in vitro study, Student t-test was used when two values were compared. Differences were considered significant when p < 0.05.
Total cholesterol circulating levels
In vivo experiments
Ex vivo endothelial function measurements
As seen in vivo, the endothelial function of the animals fed 8 weeks with the cholesterol diet was heterogeneous. In those animals hypercholesterolemia had no effect on the acetylcholine-induced relaxation of thoracic aortic rings while for the abdominal aortic sections; the response to acetylcholine was highly variable. As illustrated in Figure 6C and 6D the endothelium-independent response to sodium nitroprusside of aortic rings was normal and similar for all treatments and controls.
Our results clearly show that endothelial function can be assessed non-invasively by transcutaneous ultrasound of the abdominal aorta in hypercholesterolemic rabbits. The method was easily feasible in all animals and yielded very reproducible results. We also show that this in vivo method correlates very well with the ex vivo evaluation of endothelial function on isolated aortic rings. To our knowledge, this is the first demonstration of such a comparison.
Ultrasound imaging of the brachial artery in response to reactive hyperaemia has been used in many studies in humans [6–12]. Normal arteries dilate in response to reactive hyperaemia while arteries with an abnormal endothelial function show a decreased or absent response to this stimulus. Ultrasound evaluation of the aorta in rabbits has been performed in the past mostly to evaluate the extent of atherosclerotic plaques in response to a hypercholesterolemic diet. Intravascular ultrasound has been used to document endothelial dysfunction but has never been compared to ex vivo evaluation of endothelial function on aortic rings [1–5]. Our method is much less invasive for the animals than intravascular ultrasound. It is compatible with longitudinal studies requiring repeated measurements in the same animal and correlates very well with the ex vivo data [13, 14].
The extent of endothelial dysfunction observed after 2 weeks of hypercholesterolemic diet was surprising although this parameter has not been studied very much after such a short exposure to hypercholesterolemia in rabbits . The paradoxical contraction in response to acetylcholine signs the presence of endothelial dysfunction and this is confirmed in bath studies. However, the results obtained after 8 weeks were unexpected. Indeed, a significant number of animals had an improved endothelial function after 8 weeks of hypercholesterolemia compared to the two weeks data both in vivo and ex vivo. This transient improvement of endothelial function in the early phases of the atherosclerotic process has never been described before to our knowledge and the underlying mechanisms responsible for this paradoxical response need to be explored. This dysfunction may relate to an initial stress response of the aortic endothelium to hyperlipidemia then evolving with the development of atherosclerosis lesions.
Endothelial function can be evaluated non-invasively in rabbits using a standard vascular ultrasound probe by a trans-abdominal approach. Results correlate well with in vitro data. A transient improvement in endothelial function can occur after 8 weeks of hypercholesterolemia in some animals for reasons that remain unclear.
The authors wish to thank Mr. André Blouin for his expert technical support with the animal studies. This work was supported by grants from the Heart and Stroke Foundation of Canada (Quebec) and from the Institut de cardiologie de Québec to Jacques Couet. Jacques Couet, Marie Arsenault and Bruno Battistini are scholars from the Fonds de la recherche en santé du Québec.
- Chin HP, Liu CR, Liu CH, Blankenhorn DH: Very early aortic responses during atherosclerosis induction in rabbits: measurement by duplex ultrasound. I. Non-invasive study of aortic hyperresponsiveness to serotonin. Atherosclerosis 1990, 83: 1-8.View ArticlePubMedGoogle Scholar
- Mano T, Masuyama T, Yamamoto K, Naito J, Kondo H, Nagano R, Tanouchi J, Hori M, Inoue M, Kamada T: Endothelial dysfunction in the early stage of atherosclerosis precedes appearance of intimal lesions assessable with intravascular ultrasound. Am Heart J 1996, 131: 231-238.View ArticlePubMedGoogle Scholar
- Hiromoto M, Toma Y, Tomochika Y, Umemoto S, Matsuzaki M: Echographical assessment of the early stage of experimental atherosclerosis of the descending aorta in rabbits. Jpn Circ J 1996, 60: 691-698. 10.1253/jcj.60.691View ArticlePubMedGoogle Scholar
- Schwarzacher S, Weidinger F, Krejcy K, Raberger G: Assessment of changes in vasomotor tone in vivo using intravascular ultrasound. J Pharmacol Toxicol Methods 1992, 28: 143-147. 10.1016/1056-8719(92)90076-DView ArticlePubMedGoogle Scholar
- Ochiai R, Kisanuki A, Asada Y, Asato M, Tamura S, Sumiyoshi A: Intravascular ultrasound imaging in the assessment of atherosclerotic plaques in rabbit abdominal aorta: comparison with histologic findings. Invest Radiol 2002, 37: 309-313. 10.1097/00004424-200206000-00001View ArticlePubMedGoogle Scholar
- Ramsey M, Goodfellow J, Bellamy M, Lewis M, Henderson A: Non-invasive detection of endothelial dysfunction. Lancet 1996, 348: 128-129.View ArticlePubMedGoogle Scholar
- Sorensen KE, Kristensen IB, Celermajer DS: Atherosclerosis in the human brachial artery. J Am Coll Cardiol 1997, 29: 318-322. 10.1016/S0735-1097(96)00474-3View ArticlePubMedGoogle Scholar
- Celermajer DS, Sorensen KE, Gooch VM, Spiegelhalter DJ, Miller OI, Sullivan ID, Lloyd JK, Deanfield JE: Non-invasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis. Lancet 1992, 340: 1111-1115. 10.1016/0140-6736(92)93147-FView ArticlePubMedGoogle Scholar
- Sorensen KE, Celermajer DS, Spiegelhalter DJ, Georgakopoulos D, Robinson J, Thomas O, Deanfield JE: Non-invasive measurement of human endothelium dependent arterial responses: accuracy and reproducibility. Br Heart J 1995, 74: 247-253.View ArticlePubMedPubMed CentralGoogle Scholar
- Lambert J, Aarsen M, Donker AJ, Stehouwer CD: Endothelium-dependent and -independent vasodilation of large arteries in normoalbuminuric insulin-dependent diabetes mellitus. Arterioscler Thromb Vasc Biol 1996, 16: 705-711.View ArticlePubMedGoogle Scholar
- Kobayashi H, Yoshida A, Kobayashi M, Nakao S: Non-invasive detection of endothelial dysfunction with 30 MHz transducer. Lancet 1996, 347: 1336-1337. 10.1016/S0140-6736(96)90986-1View ArticlePubMedGoogle Scholar
- Corretti MC, Plotnick GD, Vogel RA: Technical aspects of evaluating brachial artery vasodilatation using high-frequency ultrasound. Am J Physiol 1995, 268: H1397-H1404.PubMedGoogle Scholar
- Ludwig M, Stumpe KO, Sauer A, Kolloch R, Goertz U, Vetter H: Effects of a high-cholesterol diet on arterial wall thickness and vascular reactivity in young rabbits. Clin Investig 1992, 70: 105-112.View ArticlePubMedGoogle Scholar
- Ludwig M, Stumpe KO, Sauer A, Kolloch R, Goertz U, Vetter H: Short-term high dietary cholesterol in early life induces persistent increases in arterial wall thickness and vascular reactivity to noradrenaline. J Hypertens Suppl 1991, 9: S136-S137.View ArticlePubMedGoogle Scholar
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