A total of 240 C57Bl/6 mice were randomized to one of the following prophylactic treatment arms: (A) placebo (saline; n = 60); (B) Aliskiren (50 mg/kg; n = 60); (C) Perindopril (3 mg/kg; n = 60); or (D) Valsartan (10 mg/kg; n = 60). RAS antagonists were administered orally on a daily basis for the entire study period of 13 weeks. Furthermore, mice from each prophylactic treatment arm were randomized to one of the following chemotherapeutic regimens: (i) TRZ (4 mg/kg weekly, intraperitoneal (i.p.); n = 20); (ii) DOX (4 mg/kg weekly, i.p.; n = 20); or (iii) DOX+TRZ (n = 20).