Figure 2

Acoustic intensity calibration curves of SonoVue® at room temperature and at a temperature <4°C. Acoustic intensity is presented on the Y-axis at different temperatures and at different concentrations of SonoVue®. At room temperature (red circle) there is attenuation above 1.5 mg/L; at a temperature <4°C (light blue square) attenuation occurs at a concentration between 1 mg/L and 1.5 mg/L. A linear relationship between concentration and acoustic intensity is seen below 1 to 1.5 mg/L at both temperatures.