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Systemic vascular function, measured with forearm flow mediated dilatation, in acute and stable cerebrovascular disease: a case-control study
© Beer et al; licensee BioMed Central Ltd. 2010
Received: 26 August 2010
Accepted: 19 October 2010
Published: 19 October 2010
Acute ischaemic stroke is associated with alteration in systemic markers of vascular function. We measured forearm vascular function (using forearm flow mediated dilatation) to clarify whether recent acute ischaemic stroke/TIA is associated with impaired systemic vascular function.
Prospective case control study enrolling 17 patients with recent acute ischaemic stroke/TIA and 17 sex matched controls with stroke more than two years previously. Forearm vascular function was measured using flow medicated dilatation (FMD).
Flow mediated dilatation was 6.0 ± 1.1% in acute stroke/TIA patients and 4.7 ± 1.0% among control subjects (p = 0.18). The mean paired difference in FMD between subjects with recent acute stroke and controls was 1.25% (95% CI -0.65, 3.14; p = 0.18). Endothelium independent dilatation was measured in six pairs of participants and was similar in acute stroke/TIA patients (22.6 ± 4.3%) and control subjects (19.1 ± 2.6%; p = 0.43).
Despite the small size of this study, these data indicate that recent acute stroke is not necessarily associated with a clinically important reduction in FMD.
Endothelial dysfunction, as measured by impaired brachial flow-mediated dilatation, occurs early in atherosclerosis. It is associated with subsequent intracranial small vessel disease (as manifest by small, deep, lacunar ischaemic stroke and brain imaging evidence of deep white matter infarction), and is an independent predictor of vascular events[1–4]. Moreover, endothelial dysfunction can be reversed with treatments that lower C-reactive protein and homocysteine, suggesting that systemic inflammation may mediate the observed vascular dysfunction in patients with stable cerebrovascular disease.
However, the importance of endothelial dysfunction in the pathogenesis of acute ischaemic stroke and transient ischaemic attacks (TIA) remains uncertain. Acute ischaemic stroke is associated with a rise in markers of endothelial activation in the systemic circulation [6, 7] and with fewer circulating endothelial progenitor cells  but whether these results reflect an underlying primary disturbance of endothelial function in the pathogenesis of acute ischaemic stroke, or an acute phase response to the cerebral injury, is unknown. The endothelial marker E-selectin is elevated in acute stroke and returns to normal concentrations within 3-6 months. However it is not known if endothelial function is impaired in subjects with acute stroke (compared to those who have suffered a remote stroke).
We hypothesised that acute ischaemic stroke would be associated with impairment of systemic endothelial dysfunction. Our aim was to measure systemic vascular function directly (using forearm flow mediated dilatation) in patients with acute ischaemic stroke, and controls with stable cerebrovascular disease, to clarify whether recent acute ischaemic stroke/TIA is in fact associated with impaired systemic vascular function.
Materials and methods
The research was approved by the Royal Perth Hospital Ethics Committee. All participants provided written consent.
Prospective case-control study.
Acute stroke units and TIA clinics of two university teaching hospitals in Perth, Australia.
Cases of recent acute TIA or ischaemic stroke
Patients with a clinical diagnosis of acute ischaemic stroke or TIA within the previous 10 days. All participants underwent brain imaging with computed tomography. Clinical records were reviewed subsequent to the patient's discharge to confirm a final clinical diagnosis of an acute cerebral ischaemic event and classify the clinical syndrome using the Oxfordshire Community Stroke Project classification .
Patients with blood glucose level >13 mmol/L and those with diagnosed infection or malignancy.
Control participants were patients who had a history of acute ischaemic stroke or TIA more than two years previously, had been enrolled in the VITamins TO Prevent Stroke Trial, taken part in a completed sub-study of arterial inflammation, and were placebo treated. Sex matched control subjects closest to the age of cases with acute stroke/TIA patients were selected. This resulted in sex matched controls with mean age difference of ± 1.1 years (maximum ± 7 years) from recent acute stroke/TIA subjects at the time of assessment of forearm flow mediated dilatation.
Recent Stroke/TIA n = 17
Stable Disease n = 17
Gender, n = F
Age at FMD scan, years
64.2 ± 13.0
64.7 ± 11.7
Clinical Syndrome TACS
139.9 ± 21.0
138.9 ± 18.7
DBP, mm Hg
84.1 ± 13.6
79.6 ± 14.7
3.9 ± 0.9
4.2 ± 0.9
1.1 ± 0.3
1.3 ± 0.5
2.1 ± 1.1
2.2 ± 0.6
1.3 ± 0.5
1.4 ± 0.6
Additional file 1: Set-up of semi-automated software for measurement of flow mediated dilatation. A video file, demonstrating preparation of the semi-automated system (AVI 8 MB)
Additional file 2: Acquisition of flow mediated dilatation data using semi-automated software. A sample video file, demonstrating acquisition of flow mediated dilatation data (AVI 9 MB)
Log-normal data were log transformed prior to analysis and compared using paired t tests. Non-normal data were analysed using non parametric tests. Categorical variables were assessed with the chi squared or fisher exact test. Data were analysed using SPSS 15.0
We recruited 17 patients with recent acute ischaemic stroke (n = 16) or TIA (n = 1), and 17 age- and sex-matched controls who had experienced an ischaemic stroke or TIA more than two (median 4) years previously.
Cases and controls were adequately matched, with no significant differences between the two groups in the variables evaluated. (Table 1) The presenting syndromes of the cases with recent acute ischaemic stroke, and control participants, were varied. Among the patients with recent acute stroke or TIA, 1 had a total anterior circulation syndrome, 6 had partial anterior circulation syndromes, 4 had lacunar syndromes, 2 had posterior circulation syndromes, and 4 had presented with a TIA. Control subjects had a history of TIA (n = 4) or total anterior (n = 2), partial anterior (n = 4), lacunar (n = 6) or posterior (n = 1) syndrome ischaemic stroke.
Forearm vascular function.
Acute Stroke or TIA Mean ± SEM
Stable Cerebrovascular Disease Mean ± SEM
Endothelium dependent vasodilatation
n = 17
n = 17
Baseline Diameter (mm)
3.6 ± 0.2
3.5 ± 0.2
6.0 ± 1.1
4.7 ± 1.0
Endothelium independent vasodilatation
n = 6
n = 6
GTN-mediated dilatation (%)
22.6 ± 4.3
19.1 ± 2.6
Mean blood pressure after the administration of GTN to 6 subjects with recent acute stroke/TIA was 144.4/85.6 mmHg, which did not differ significantly from the baseline mean systolic (152.2 mmHg; p = 0.34) or diastolic (87.2 mmHg, p = 0.83) blood pressures among this group. No adverse events related to the administration of GTN were observed.
These data suggest that vascular function, measured with FMD, is similar in patients with recent acute and stable cerebrovascular disease. If anything, FMD tended to be better in subjects with acute stroke, which was an unexpected finding. These data do not support the hypothesis that acute stroke is associated with worsening of systemic vascular function. The lower limit of the 95% confidence interval of the mean paired differences between subjects with recent acute stroke and controls with prior cerebrovascular disease was -0.65%. This effectively excludes a worsening of FMD associated with acute ischaemic stroke of a magnitude which would be considered clinically important (>2%).
Currently, concern regarding the safety of administration of GTN to people with acute ischaemic stroke precludes full measurement of FMD in acute stroke patients outside research protocols. Our preliminary data suggest that GTN mediated FMD can be safely assessed in people with recent acute ischaemic stroke. Further work in this population would ideally assess flow and GTN mediated dilatation, to ensure any observed differences are endothelium dependent (rather than due to differences in smooth muscle function, for example related to statin therapy). Safety of assessment of FMD in the acute phase of stroke could be confirmed by continuous BP monitoring whilst GTN is administered, combined with assessment of cerebral perfusion.
Results in context of other studies
Our findings are consistent with other data suggesting that endothelial function is not necessarily worse in subjects with recent acute stroke compared to subjects with stable disease. For example, individuals with symptomatic ICA stenosis and severe periventricular white matter lesions had higher levels of VCAM-1 (a marker of endothelial cell activation) than subjects with acute stroke. We are aware of only one other study that has assessed FMD in patients with acute stroke. Those data were collected to test the hypothesis that endothelial dysfunction contributes to the risk of arterial stroke to a greater extent than embolic stroke, and thus did not utilize healthy or disease controls. Patients hospitalised with ischaemic stroke and atrial fibrillation within 5-10 days (n = 20) were found to have less impaired vascular function (5.7 ± 3.3%) than patients with ischaemic stroke in sinus rhythm (n = 32; 3.2 ± 2.4%; p = 0.041). Future work should stratify participants according to stroke sub-type. FMD has been found to be associated with survival free from cardiac events, and composite cardiovascular outcomes. Although our data suggest that FMD does not differ significantly between patients with acute stroke and stable cerebrovascular disease, future work should determine whether the degree of impairment of FMD is associated with future cerebrovascular events.
Strengths of our study
To our knowledge this is the first study comparing forearm vascular function in people with recent acute ischaemic stroke or TIA and controls with stable cerebrovascular disease. Inclusion of a control population, sharing similar risk factor and treatment profiles to the cases with acute ischaemic stroke/TIA, is a strength of the study. Our aim was to determine the association of acute stroke with vascular function, and we thus chose to control for background cerebrovascular disease and treatments. However the study would have been furthered strengthened by the simultaneous inclusion of a healthy control population. Our methodology is also likely to be reliable, as the measurements were made by a single observer using semi-automated edge detection software. Forearm flow mediated dilatation (FMD) is a validated non invasive marker of systemic endothelial function and a functional assay of endothelium-derived nitric oxide.
The major weakness of our study is the potential for random error because of the small number of patients and controls studied. Because of the small data set, the results may be falsely negative. Further, there is potential for selection bias as the cases and controls were selected from hospital and clinical trial populations respectively. Interpretation of our data is limited by the heterogenous nature of the participants with acute stroke/TIA. It might be expected, for example, that participants with lacunar or embolic stroke are less likely to show evidence of vascular dysfunction than participants with large artery embolic stroke. Our participants were heavily pre-treated which may influence the observed results, given that commonly used medications, such as statins, may improve vascular function and reduce the risk of stroke[17, 18]. We also enrolled patients up to 10 days after symptom onset and thus may have failed to ascertain differences in the very early phase of acute stroke. Future work should enrol patients in the very early phase after stroke (ideally within first 24 hours of ictus).
Despite the small size of this study, these data indicate that recent acute stroke may not necessarily be associated with a clinically important reduction in FMD.
These data suggest that subjects with stroke more than two years previously have similar vascular function to subjects with recent stroke. The clinical utility of assessment of FMD in subjects with recent acute ischaemic stroke thus remains uncertain.
This study was funded by the Centre for Training in Clinical Cerebrovascular and Cardiovascular Research, a National Health and Medical Research Council funded Centre of Clinical Research Excellence. The authors thank Ms Lisa Rich (Research Assistant) for the provision of sample videos.
- Hoth KF, Tate DF, Poppas A, Forman DE, Gunstad J, Moser DJ, Paul RH, Jefferson AL, Haley AP, Cohen RA: Endothelial function and white matter hyperintensities in older adults with cardiovascular disease. Stroke. 2007, 38 (2): 308-312. 10.1161/01.STR.0000254517.04275.3f.View ArticlePubMedPubMed CentralGoogle Scholar
- Kim JS, Lee HS, Park HY, Kim SS, Kang HG, Kim NH, Park JS, Kim Y: Endothelial function in lacunar infarction: a comparison of lacunar infarction, cerebral atherosclerosis and control group. Cerebrovasc Dis. 2009, 28 (2): 166-170. 10.1159/000226115.View ArticlePubMedGoogle Scholar
- Pretnar-Oblak J, Sabovic M, Pogacnik T, Sebestjen M, Zaletel M: Flow-mediated dilatation and intima-media thickness in patients with lacunar infarctions. Acta Neurol Scand. 2006, 113 (4): 273-277. 10.1111/j.1600-0404.2006.00578.x.View ArticlePubMedGoogle Scholar
- Yeboah J, Crouse JR, Hsu FC, Burke GL, Herrington DM: Brachial flow-mediated dilation predicts incident cardiovascular events in older adults: the Cardiovascular Health Study. Circulation. 2007, 115 (18): 2390-2397. 10.1161/CIRCULATIONAHA.106.678276.View ArticlePubMedGoogle Scholar
- Chan YH, Lau KK, Yiu KH, Li SW, Chan HT, Fong DY, Tam S, Lau CP, Tse HF: Reduction of C-reactive protein with isoflavone supplement reverses endothelial dysfunction in patients with ischaemic stroke. Eur Heart J. 2008, 29 (22): 2800-2807. 10.1093/eurheartj/ehn409.View ArticlePubMedGoogle Scholar
- Cherian P, Hankey GJ, Eikelboom JW, Thom J, Baker RI, McQuillan A, Staton J, Yi Q: Endothelial and platelet activation in acute ischemic stroke and its etiological subtypes. Stroke. 2003, 34 (9): 2132-2137. 10.1161/01.STR.0000086466.32421.F4.View ArticlePubMedGoogle Scholar
- Lip GYH, Blann AD, Farooqi IS, Zarifis J, Sagar G, Beevers DG: Sequential alterations in haemorheology, endothelial dysfunction, platelet activation and thrombogenesis in relation to prognosis following acute stroke: The West Birmingham Stroke Project. Blood Coagulation & Fibrinolysis. 2002, 13 (4): 339-347.View ArticleGoogle Scholar
- Ghani U, Shuaib A, Salam A, Nasir A, Shuaib U, Jeerakathil T, Sher F, O'Rourke F, Nasser AM, Schwindt B, et al: Endothelial progenitor cells during cerebrovascular disease. Stroke. 2005, 36 (1): 151-153. 10.1161/01.STR.0000149944.15406.16.View ArticlePubMedGoogle Scholar
- Bamford J, Sandercock P, Dennis M, Burn J, Warlow C: Classification and natural history of clinically identifiable subtypes of cerebral infarction. Lancet. 1991, 337 (8756): 1521-1526. 10.1016/0140-6736(91)93206-O.View ArticlePubMedGoogle Scholar
- VITATOPS the VITAmins TO Prevent Stroke trial: rationale and design of a randomised trial of B-vitamin therapy in patients with recent transient ischaemic attack or stroke (NCT00097669) (ISRCTN74743444). International Journal of Stroke. 2007, 2 (2): 144-150. 10.1111/j.1747-4949.2007.00111.x.Google Scholar
- Potter K, Lenzo N, Eikelboom JW, Arnolda LF, Beer C, Hankey GJ: Effect of long-term homocysteine reduction with B vitamins on arterial wall inflammation assessed by fluorodeoxyglucose positron emission tomography: a randomised double-blind, placebo-controlled trial. Cerebrovasc Dis. 2009, 27 (3): 259-265. 10.1159/000199463.View ArticlePubMedGoogle Scholar
- Woodman RJ, Playford DA, Watts GF, Cheetham C, Reed C, Taylor RR, Puddey IB, Beilin LJ, Burke V, Mori TA, et al: Improved analysis of brachial artery ultrasound using a novel edge-detection software system. J Appl Physiol. 2001, 91 (2): 929-937.PubMedGoogle Scholar
- Sorensen KE, Celermajer DS, Spiegelhalter DJ, Georgakopoulos D, Robinson J, Thomas O, Deanfield JE: Non-invasive measurement of human endothelium dependent arterial responses: accuracy and reproducibility. Br Heart J. 1995, 74 (3): 247-253. 10.1136/hrt.74.3.247.View ArticlePubMedPubMed CentralGoogle Scholar
- de Leeuw FE, de Kleine M, Frijns CJ, Fijnheer R, van Gijn J, Kappelle LJ: Endothelial cell activation is associated with cerebral white matter lesions in patients with cerebrovascular disease. Ann N Y Acad Sci. 2002, 977: 306-314. 10.1111/j.1749-6632.2002.tb04831.x.View ArticlePubMedGoogle Scholar
- Chlumsky I, Charvat J: Endothelial dysfunction, distensibility and intima-media thickness and aetiology of stroke. J Int Med Res. 2005, 33 (5): 555-561.View ArticlePubMedGoogle Scholar
- Takase B, Matsushima Y, Uehata A, Ishihara M, Kurita A: Endothelial dysfunction, carotid artery plaque burden, and conventional exercise-induced myocardial ischemia as predictors of coronary artery disease prognosis. Cardiovasc Ultrasound. 2008, 6: 61-10.1186/1476-7120-6-61.View ArticlePubMedPubMed CentralGoogle Scholar
- Dupuis J, Tardif JC, Cernacek P, Theroux P: Cholesterol reduction rapidly improves endothelial function after acute coronary syndromes. The RECIFE (reduction of cholesterol in ischemia and function of the endothelium) trial. Circulation. 1999, 99 (25): 3227-3233.View ArticlePubMedGoogle Scholar
- Waters DD, Schwartz GG, Olsson AG, Zeiher A, Oliver MF, Ganz P, Ezekowitz M, Chaitman BR, Leslie SJ, Stern T: Effects of atorvastatin on stroke in patients with unstable angina or non-Q-wave myocardial infarction: a Myocardial Ischemia Reduction with Aggressive Cholesterol Lowering (MIRACL) substudy. Circulation. 2002, 106 (13): 1690-1695. 10.1161/01.CIR.0000031568.40630.1C.View ArticlePubMedGoogle Scholar
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