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Automated quantification of left atrial size using three-beat averaging real-time three dimensional Echocardiography in patients with atrial fibrillation
© Heo et al. 2015
Received: 13 June 2015
Accepted: 13 August 2015
Published: 25 August 2015
Left atrial (LA) sizing in patients with atrial fibrillation (AF) is crucial for follow-up and outcome. Recently, the automated quantification of LA using the novel three-beat averaging real-time three dimensional echocardiography (3BA-RT3DE) is introduced. The aim of this study was to assess the feasibility and accuracy of 3BA-RT3DE in patients with atrial fibrillation (AF).
Thirty-one patients with AF (62.8 ± 11.7 years, 67.7 % male) were prospectively recruited to have two dimensional echocardiography (2DE) and 3BA-RT3DE (SC 2000, ACUSON, USA). The maximal left atrial (LA) volume was measured by the conventional prolate-ellipse (PE) and area-length (AL) method using three-beat averaging 2D transthoracic echocardiography and automated software analysis (eSie volume analysis, Siemens Medical Solution, Mountain view, USA); measurements were compared with those obtained by computed tomography (CT).
Maximal LA volume by 3BA-RT3DE was feasible for all patients. LA volume was 68.4 ± 28.2 by PE-2DE, 89.2 ± 33.1 by AL-2DE, 100.6 ± 31.8 by 3BA-RT3DE, and 131.2 ± 42.2 mL by CT. LA volume from PE-2DE (R2 = 0.48, p < 0.001, ICC = 0.64, p < 0.001), AL-2DE (R2 = 0.47, p < 0.001, ICC = 0.67, p < 0.001), and 3BA-RT3DE (R2 = 0.50, p = 0.001, ICC = 0.65, p < 0.001) showed significant correlations with CT. However, 3BA-RT3DE demonstrated a small degree of underestimation (30.5 mL) of LA volume compared to 2DE-based measurements. Good-quality images from 3BA-RT3DE (n = 16) showed a significantly tighter correlation with images from CT scanning (R2 = 0.60, p = 0.0004, ICC = 0.76, p < 0.001) compared to those of fair quality.
Automated quantification of LA volume using 3BA-RT3DE is feasible and accurate in patients with AF. An image of good quality is essential for maximizing the value of this method in clinical practice.
Left atrial (LA) size has been demonstrated as an important factor in atrial fibrillation (AF) development [1, 2]. In patients with a diagnosed AF, LA enlargement is related with a cerebrovascular outcome,  a risk of AF relapse after electrical cardioversion [4, 5] or catheter ablation [6, 7].
Therefore, accurate assessment of LA size is critical for making prognostic and treatment decisions in patients with AF. Transthoracic echocardiography (TTE) is the most common method for assessing LA volume . However, data obtained from 2D echocardiography (2DE) are limited due to geometric assumptions and foreshortening of the LA cavity. LA remodeling is frequently asymmetrical, rendering standard geometric assumptions even more inadequate . Therefore, three-dimensional assessment of LA might help to solve this issue. In addition, current recommendations suggest multi-beat measurements of LA volume in AF patients, which further increases the potential variability of 2DE images [9, 10].
Three-dimensional echocardiography (3DE) exhibits accurate assessment of LA volume and better intra-observer and inter-observer agreement when compared to those obtained with magnetic resonance imaging (MRI) [11, 12] or computed tomography (CT) [13, 14]. However, previous studies often excluded AF patients [11, 14–17]. Recently, a novel automated three-beat averaging real-time three-dimensional echocardiography (3BA-RT3DE) method was introduced . In this study, we examined the feasibility and accuracy of the 3BA-RT3DE to measure LA volume in patients with stable AF compared to that of 2DE and CT.
Materials and methods
Thirty-one consecutive patients with AF were prospectively recruited. All patients were stable without any changes in clinical condition or treatment between studies. Inclusion criteria were ECG-documented AF with an echo window that was at least fair in 2DE. Exclusion criteria included decompensated heart failure, tachycardia (heart rate over 100 beats per minute), prior valve replacement surgery, and history of renal failure. Patients received 2DE, 3BA-RT3DE, and cardiac 64-slice multi-detector CT scans. The median interval between CT and echocardiography was two days (IQR 0–7 days). And 74.2 % of CT scans were done within one week. Informed consent was obtained from all patients, and the institutional review board of Severance Hospital of Yonsei University approved the study protocol.
Echocardiographic image acquisition and quantification
Two experienced cardiologists participated in the analysis of 3BA-RT3DE. Each observer had analyzed 30 cases each before analyzing the patients in this study. Intra- and inter-observer agreement for 3BA-RT3DE was assessed using repeated measurements of all patients. The second observer used the same data sets for offline analysis as the first observer but was blinded to the results or identities of the subjects. Both observers were blinded to the results from other modalities.
CT imaging protocol
Cardiac CT scans were performed using a dual-source CT scanner (SOMATOM Definition Flash; Siemens Healthcare, Forchheim, Germany) during a single breath-hold. Imaging protocol included the administration of beta-blocker, if the baseline heart rate was above 65 beats per minute, if contraindications were absent. Heart rates during acquisition did not differ significantly between echocardiography (mean, 76.9 ± 20.1 beats/min; range, 42–120 beats/min) and CT (mean, 65.7 ± 16.6 beats/min; range, 41–136 beats/min). Optimal delay times for maximal LA volume were determined after a bolus injection of 10 ml of iopamidol (Pamiray; 370 mg of iodine/ml, Dongkook Pharma, Seoul, Korea) followed by 20 ml of saline at 5 ml/s. All CT scans were performed using the triple-phase injection method (70 ml of iomadidol followed by 30 ml of 30 % blended iopamidol with saline and 20 ml of saline at 5 ml/s). For atrial volume measurement, ECG-gated axial acquisition targeting end-systolic phase using the absolute delay method (a fixed time delay after the R wave) or maximal LA volume was utilized. Tube voltage and tube current were chosen using automatic tube potential selection software (Care kV; Siemens Healthcare, Forchheim, Germany). Images were reconstructed with a slice thickness of 0.75 mm and a reconstruction increment of 0.5 mm using sonogram-affirmed iterative reconstruction (SAFIRE; Siemens Healthcare, Forchheim, Germany) technique. CT images were uploaded into volume-rendering software (Aquaris iNtuition Edition V4.4.11, TeraRecon, San Mateo, CA, USA) and LA volume was automatically or semi-automatically segmented on the basis of the 3-dimensional threshold method, and atrial volumes were obtained after the manual exclusion of the pulmonary veins and vena cava as described in previous studies [20, 21].
Normality of continuous variables was assessed by the Kolmogorov-Smirnov test. Continuous data are presented as mean ± standard deviation. Categorical data are expressed as absolute numbers or percentages. Independent t-test was used to compare subgroups. Paired t-test was performed to compare values from two methods. Linear regression analysis was performed to evaluate relationships between methods. Values from different techniques were compared using intra-class correlation coefficient (ICC). Bland-Altman analysis was performed to evaluate differences in maximal LA volume between different techniques. Inter-observer variability was assessed by Bland-Altman analysis and ICC. A P value < 0.05 was considered statistically significant. SPSS version 16 (SPSS, Inc., Chicago, IL) was used for statistical testing.
Baseline characteristics of patients
(n = 31)
(n = 16)
(n = 15)
62.8 ± 11.7
66.5 ± 10.5
58.8 ± 11.8
Male gender (%)
166.6 ± 8.7
166.0 ± 7.9
167.2 ± 9.7
65.3 ± 13.3
63.1 ± 13.5
67.7 ± 13.0
Systolic BP (mmHg)
129.0 ± 20.1
133.4 ± 21.8
124.4 ± 17.7
Diastolic BP (mmHg)
78.7 ± 12.9
77.4 ± 13.3
80.1 ± 12.8
Heart rate (bpm)
76.9 ± 20.1
75.9 ± 20.2
77.9 ± 20.6
49.9 ± 5.4
48.6 ± 4.2
51.2 ± 6.3
34.0 ± 6.2
32.9 ± 4.0
35.1 ± 7.9
LV EF (%)
61.9 ± 11.0
61.9 ± 8.3
61.8 ± 13.6
Maximal left atrial volume (mL) using different modalities and comparison of it by echocardiography to that by computed tomography
Mean ± SD
Correlation with maximal LA volume by CT
68.4 ± 28.2
89.2 ± 33.1
100.6 ± 31.8
131.2 ± 42.2
Comparison of maximal left atrial volume (mL) using real-time three-dimensional echocardiography in good and fair image quality group
Mean ± SD
Correlation with maximal LA volume by CT
99.4 ± 29.6
101.9 ± 35.0
In this study, a novel automated 3BA-RT3DE was feasible in all patients and showed fair correlation and a lower degree of underestimation compared to 2DE, with CT scanning as the comparison modality. Furthermore, in images of good quality, maximal LA volume measured by 3BA-RT3DE showed better correlation and less variability compared to that of images with fair quality.
Many studies have compared LA volume by 3DE with 2DE,  CT [13, 14] or MRI [11, 12, 17, 23]. These published results show that 3DE yields reduced variability and higher or similar accuracy to that of 2DE, mainly because 3DE addresses some of the limitations of 2DE, such as geometric assumption [22, 24–27]. Moreover, 3DE measurements of LA volumes have clinical value for assessing response to therapy and for predicting clinical events in patients with severe left ventricular dysfunction [24, 28, 29]. However, consistent underestimation was shown in most studies, because of ambiguous endocardial borders produced by apical imaging in the far field of the ultrasound beam. Previous studies using 3DE for LA volume quantification used a semi-automated endocardial tracking algorithm that was originally developed for the quantification of left ventricular size and function [14, 22, 23]. However, this 3DE direct volumetric algorithm was validated by MRI and has good comparability to the reference modality . One study used RT3DE with software dedicated to the analysis of LA volume and demonstrated similar results to those of our study . In that study, LA volume was more accurately determined with RT3DE than 2DE, and there was a trend toward increased bias in patients with enlarged atria. However, AF patients were excluded from that study, and manual endocardial tracing was required .
Recently, two studies were conducted in AF patients to validate the RT3DE-based LA volume quantification with CT  or MRI  as a reference method. Rohner et al. showed LA volumes and ejection fraction as assessed by RT3DE compared to those obtained by CT. Although RT3DE showed a trend toward underestimating LA volume, it correlated strongly with CT measurements. Furthermore, there was robust inter- and intra-observer variability . However, 85.3 % of patients were described as being in sinus rhythm on baseline, and it only used one-beat RT3DE for LA measurement, which is not concordant with the guideline. In the other study, 2DE and RT3DE were compared with MRI for abilities to assess LA volume. RT3DE showed a moderate improvement in accuracy and the narrowest limits of agreement compared to that of 2DE; further, LA volume was underestimated by echocardiography-based methods compared with MRI. However, it also selected single-beat for evaluation of LA volume . In our study, echocardiography-based measurements of maximal LA volume showed significant correlations and similar degrees of underestimation compared with those from CT, as previously published . However, 3BA-RT3DE demonstrated the lowest degree of underestimation and the narrowest limit of agreement. Furthermore, we demonstrated that image quality is an important factor for LA volume assessment by 3DE in patients with AF. The correlation and degree of underestimation was significantly higher in images of good quality compared with images of fair quality. This is an important consideration for clinical practice, as many patients with AF exhibit poor or fair echo windows. Thus, RT3DE may be the appropriate method for measuring LA volume in patients with AF. The other potential explanation for LA volume underestimation is that LA volume may be overestimated by CT. A recent study by Agner et al. investigated LA volume by volumetric 2DE, CT, and MRI. In that study, CT overestimated LA volume compared to MRI in patients with permanent AF, whereas TTE significantly underestimated LA volume compared to CT and MRI . This difference might be related to volume effects of the contrast and saline chaser. In addition, the administration of a beta blocker to control heart rate might affect LA volume. Furthermore, although CT and MRI showed excellent intra- and inter-observer agreements, these might not be practical approaches for daily clinical practice. Currently, MRI takes approximately 60 min for image acquisition. CT scanning exposes patients to radiation regardless of the dose. The risk of renal injury is also high in patients with AF, as they often have renal disease. Patients with AF should be followed regularly for LA volume assessment; for this purpose, echocardiographic methods are widely available. However, the current recommendation of measuring at least 5 beats for LA volume is rather time-consuming. Thus, 3BA-RT3DE is a promising tool for LA assessment in AF patients, particularly for images of good quality. Further studies in larger populations of patients with diverse pathologies will allow the range of applicability of 3DE-based LA volume quantification to be carefully assessed.
Our study has limitations. We only examined maximal LA volume, which is strongly supported as a metric for assessing cardiovascular risk. However, some studies suggest that other parameters, such as minimal LA volume and phasic changes of LA, are also related to cardiovascular prognosis [28, 34–37]. These parameters might provide incremental information. In addition, although 3BA-RT3DE showed improved accuracy for LA volume measurements compared to 2DE, the clinical significance remains unclear due to the lack of prognostic information. Thus, further studies are needed to understand the prognostic value of RT3DE-based LA assessment in AF patients. Moreover, we used beta-blocker during the CT scan, which might have affected the hemodynamics of LA volume. However, our population was composed of AF patients; hence, the heart rate variability and potential use of beta-blocker were innate issues of these patients.
Automated quantification of LA volume using 3BA-RT3DE is feasible and accurate in patients with AF. An image of good quality is essential for maximizing the value of this method in clinical practice. This technique might advance and enhance the integration of RT3DE into routine clinical practice.
Geu-Ru Hong – Research Support from Siemens Medical Solution.
Joel Mancina - Employee of Siemens Medical Solution.
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